Beer spoilage associated with Pediococcus spp

Beer spoilage associated with Pediococcus spp Introduction In average lager fermentation gradual decrease of pH and specific gravity is expected while ethanol concentration is increasing. Some flavours are also formed during fermentation as esters and other flavour compounds are formed. They can remain in beer or be gradually removed either due to evaporation or further metabolism of yeast. Deviations from the average pattern of changes can signalise a contamination. Fermenting wort is therefore routinely tested for its gravity, pH and also for flavour and odour. In a sample tested rapid decrease of pH at the end of fermentation was noted. pH reached 3.7 which was well below expected 4.1 (Hough 2001). Such significant fall suggested bacterial contamination. Two groups of bacteria may cause lowering of pH. These are Acetic Acid Bacteria and Lactic Acid Bacteria. pH decrease was noted late in fermentation when no oxygen was available suggesting Lactic Acid Bacteria. Strong diacetyl odour (sweet-buttery), turbidity and time indicated Pediococcus spp., probably Pediococcus damnosus since it is the most common beer-spoiling bacterium in its genus . The spoilage characteristic associated with Pediococcus spp. Lactic Acid Bacteria are the most common contaminant in fermented wort and beer. They can be responsible for up to 90% of microbial beer spoilage incidents (Taskila et al. 2009). Within them two groups are recognised as the most common contaminants: Lactobacillus and Pediococcus. The symptoms of bacterial contamination by member of any of those two genera are similar: lowered acidity (giving to the beer sour taste) caused by production of lactic acid by the bacteria and diacetyl (buttery) off-flavour. The latter one is the main reason why pediococcal contamination is so unwanted. According to Whiting (1992) as few as 20,000 bacterial cells per 1ml can produce diacetyl in concentration of 0.36mg/L, which is 3 times higher than the taste threshold. Spoilage by Pediococcus is often characterised by ropines, but some of Lactobacilli can also give similar symptoms. Presence of any of those lactic acid bacteria negatively influences yeast performance and health slowing down fermentation (Priest 2006). Further investigation is required to determine spoilage microorganism, the cause of contamination and methods of removing unwanted bacteria from the system. Pediococci are spherical, gram positive bacteria that often form tetrads, but also may appear in pairs (Priest 2006). Generally they are catalase negative, but in low glucose medium they can produce pseudocatalase that can also break hydrogen peroxide, which may lead to false catalase test results (Priest 2003). They do not form spores and are nonmotile. The main product of their metabolism is lactic acid (homofermentative bacteria) and thought they are anaerobic they can tolerate presence of oxygen (Priest 2003). The species that can inhabit fermenting wort and beer are hop resistant. Following species have been isolated from beer: Pediococcus damnosus (formerly in brewing literature also referred to as P. cerevisiae), which is found in beer, late fermentation and brewing yeast (also wine) and is thought to be responsible for 90% of all spoilage incidents caused by Pediococci (Priest 2003, Whiting et al. 1992, ). Pediococcus inopinatus found in beer, brewing yeast, vegetables, wine, milk (Priest 2003). Pediococcus dextrinicus (Priest 2003). Pediococcus pentosaceus (Priest 2003). Pediococcus claussenii (Priest 2006). Isolation and confirmation of the presence of Pediococcus spp. To detect presence of Pediococcus species in wort it should be first filtrated (volume 100ml). A 0.2 0.45 microns membrane filter is recommended. (Lewis and Bamforth 2006). Colonies should be incubated then on suitable medium (e.g. MRS[1]with Actidione to suppress yeast growth, Raka-Ray or NBB medium) for 5 days in temperature 25oC in anaerobic conditions (Lewis and Bamforth 2006, Briggs et al. 2004). Grown bacteria can be Gram-stained and examined under microscope. Gram-positive cocci organised in tetrads suggest Pediococcus spp. although that should be checked by further tests: Catalase test should be negative no bubbles formed after dripping 3% H2O2 solution onto colony. Colonies growing have sour odour. No gas should be produced from glucose using Gibson and Abd-el-Malek method (Priest 2003, p. 211). An improved methodology for the recovery of Pediococcus spp. Traditional methods of identifying bacteria are very time consuming. The results are too slow for commercial requirements and may result in dispatching a product that does not meet health and safety criteria. Therefore rapid testing methods were developed to identify spoilage microorganisms. There are used in diagnostic tests, often designed specifically for a given industry, such as LightCycler foodproof Beer Screening Kit. The LightCycler foodproof Beer Screening Kit is based on polymerase chain reaction (PCR). In order to carry on the test sample has to be filtrated and inoculated into enriching broth as the count of about 1000 cells/ml is required to increase reliability of the result. The sample is then centrifuged, cells are lysed and DNA extracted, amplified and identified. The test can detect 24 most common species of beer spoilage bacteria of genera Lactobacillus, Pediococcus, Pectinatus and Megasphaera and identify Pediococcus damnosus as well as P. inopinatus, Lactobacillus brevis, L. lindneri and Megasphaera cerevisiae (Biotecon 2009). The method is very quick in compare to traditional methods (2 days versus up to 14) but is much more expensive. It requires three more kits and specialist equipment to carry the test and read the results, therefore can not be applied in small breweries. Solution Pediococcus damnosus was found in the fermenting wort and in beer. Special cleaning regime was employed with use of antibiotics to remove contamination as well as acid wash was implemented to pitching yeast. References BIOTECON. 2009. http://bc-diagnostics.de/?cid=1201264657lang=1name=foodproof+Beer+Screening+Kit%2C+Hybridization+Probes+(LC+1.x)+ http://www.bc-diagnostics.com/public/DB_Data/files/Downloads/BeerScreening.pdf leaflet. Biotecon Diagnostic GmbH. Potsdam. Briggs, D.E., Boulton, C.A., Brookes, P.A., Stevens, R. (2004). Microbiology. In Brewing. Science and Practice. pp 606 649. Woodhead Publishing Limited and CRC Press, LTD. Cambridge. Hough, J.S., (2001). The Biotechnology of Malting and Brewing. Cambridge University Press. Melbourne. Lewis, M.J., Bamforth, C.W., (2006). Microbiology. In Essays in Brewing Science. pp. 58 68. Springer Science+Business Media LLC. New York. Priest, F.G., (2003). Gram-positive Brewery Bacteria. In Brewing Microbiology. 3rd ed. (ed. by Priest, F.G. and Campbell, I.). pp 181 218. Kluwer Academic/Plenum Publishers. New York. Priest, F.G., (2006). Microbiology and Microbial Control in the Brewery. In Handbook of brewing. 2nd ed. (ed. by Priest, F.G. and Stewart, G.H.). pp 607 628. Tylor and Francis. Boca Roca. Taskila, S., Nebauer, P., Tuomola, M., Breinstein, A., Kronlà ¶f, J., Hillukkala, T. (2009). Improved Enrichment Cultivation of Beer Spoiling Lactic Acid Bacteria by Continuos Glucose Addition. In Journal of Institute of Brewing. Vol. 15. No 3. 177-182. Whiting, M., Crichlow, M., Ingledew, W.M., Ziola, B., (1992). Detection of Pediococcus spp. In Brewing Yeast by a Rapid Immunoassay. In Applied and Environmental Microbiology. Vol. 58. No 2. 713-716

Internet Applications In Business :: essays research papers fc

The Benefits of Businesses that use the Internet Almost all national retail organizations today have an Internet site where anyone can place an order. Most are even able to accept credit card payment for instant processing of your order. In fact, there are some companies that accept orders solely from the Internet. Office Depot has gone one step further than the competition; they have included perks for their contract customers also. Specifically, they have linked the Web-site to their ES9000 mainframe enabling customers to get their own specific contract pricing. The closest any competitors have come is storing a customer’s shipping information. The result was $67 Million in sales last year. That doesn’t come close to an Internet only retailer like Amazon.com, but it is a phenomenal success for Depot’s first year in the Internet market. Increased sales revenue is not the only benefit that this marketing plan has reaped. A new level of efficiency has also been reached. The leading benefit that has created such efficiency is the number of customer orders that Depot does not have to: 1.  Ã‚  Ã‚  Ã‚  Ã‚  Take up more of a salesperson’s time on the phone. 2.  Ã‚  Ã‚  Ã‚  Ã‚  Take up an order-entry clerk. 3.  Ã‚  Ã‚  Ã‚  Ã‚  Waste time trying to understand precisely what the customer needs. (The customer can browse though our entire catalog of merchandise) Instead, the company’s time can be spent on more important customer service issues. A salesperson can get out in the field to meet one-on-one with their customers. Order-entry clerks can spend more time making sure they key exactly what the customer wants. That covers the benefits to the company, but how about the customer. Are they benefiting as well? Customer feedback has been overwhelmingly positive. This is mainly true because Internet ordering is an additional service, not a replacement of service. Some customers in the past would complain that they couldn’t speak to a Real Person when they needed to most. Now when they need to place an order, there is no need to speak to someone unless more clarification is required, and when it is, there are more Real People available to speak with. Company Email Though some people think that the Email account that their company supplies them is private and confidential, it is actually neither. Most companies explain their Email policy in great detail, but one would have to look for it in their Employee Manual on their own. One of the main reasons why companies do this is to protect themselves legally.

Hemingway :: essays research papers

From the time Ernest Hemingway became a renowned author, his works, as well as his life, have been analyzed by many. Under such scrutiny, many aspects of Hemingway’s works and life experiences have been in question to the realities and fallacies, which he laid forth. Much of Hemingway’s life, especially his time volunteering as an ambulance driver in Europe, has been in question to the true validity of his myth as a true adventurer and hero. However, as I have found, much of the mythology surrounding Hemingway is very true indeed, which leads me to believe that he did not embellish his life but rather used his experiences to create some of the greatest works of literature to be written throughout the twentieth century.   Ã‚  Ã‚  Ã‚  Ã‚  One of Ernest Hemingway’s greatest novels, â€Å"A Farewell to Arms†, has been surrounded by controversy among literary, as well as historical, scholars regarding Hemingway’s inspiration for the famous novel. Many feel that Ernest Hemingway created this fictional book solely from his imagination rather than his experiences, while others believe that Hemingway drew the inspiration for this book from his experience as a volunteer ambulance driver throughout the war. Even though there has been much controversy, there is documented historical proof that the experiences that Hemingway had experienced from his time in the war had influenced his creation of â€Å"A Farewell to Arms†.   Ã‚  Ã‚  Ã‚  Ã‚  One of the most prevalent sources for Ernest Hemingway’s inspiration for â€Å"A Farewell to Arms† can be found in his relationship with ( ?Anges, the Amer. Nurse), who was a love interest and nurse at the hospital that Hemingway recuperated at after being injured by machine gun fire. The relationship that was created between Hemingway and (Anges) led to the inspiration and creation of one of the main character in â€Å"A Farewell to Arms†, as Mrs. Catherine Barkley. The similarities between these two characters of Hemingway’s life, one real and the other fictitious, is astonishing. For instance, both Catherine Barkley and (Anges) where pretty American nurses during a war, while both also being in love with a wounded ambulance driver at their hospitals.   Ã‚  Ã‚  Ã‚  Ã‚  The similarities between the people of Hemingway’s life and his fictitious characters can also be found between Lieutenant Henry, the main character of â€Å"A Farewell to Arms†, and Hemingway himself. Once again, the similarities between these characters is astonishing; so much that Lieutenant Henry seems to be Hemingway’s idea of his younger self rather that a fictitious character.

Help Stop Bullying Essay

An 11 year old Massachusetts boy, Carl Joseph Walker-Hoover, hung himself April 6, 2009 because he was being taunted daily for being gay. The mother begged the school to address the problem, but nothing was ever done about it and now a woman is left son less. That was at least the fourth suicide of a middle-school aged child linked to bullying that year. Most harassment and bullying go unreported. Children are being bullied each and every day, but the question is what should be done to stop it? Each and every day a child is being teased or picked on because of the way they look, act, dress, or because of what they believe in. Children should not have to be tortured just because they are not like everyone else. Children should be able to come to school and enjoy themselves and not worry about being picked on. A child that is being bullied can have many problems such as low self esteem and low self confidence which could lead them to suicide. Also, children that are bullied may start doing poorly in school or maybe even drop out because they do not feel safe at school. Bullying really needs to be stooped because it can leave a child physically, emotionally, and mentally scarred for the rest of their lives. Some schools say that there is very little that they can do about bullying because a lot of bullying starts off of school property. â€Å"Much of today’s bullying originates online, school officials said, with the growing use of social networking websites and cell phones has led to new ways to intimidate and tease. But because cyber bullying usually happens off-campus school administrators have little recourse† (Nissley 2010). I do not agree with the school district when they say that there is little they can do. Even though, it may start online, it continues or become worse at the school. With that being said, they need to try and do more so it can stop all together no matter where it starts. The solution that they think will help stop bullying is if the educate student about it more. Like Nissley (2010) stated, â€Å"In a model that has been mirrored by other school districts across the state, Abington Heights has been working on educating students as early as kindergarten about bullying and their responsibility to report it when they see it (p. 1). I think that it is good to educate the students about bullying and reporting it, but what is that really going to do? As we all know a lot of students will not tell because they will be too afraid. Or if they do tell they will be little done about it and they will continue to get bullied. So, their solution would not be effective at all. â€Å"Safe to learn: Embedding anti-bullying work in schools is the overarching anti-bullying guidance for schools and was launched in 2007† (teacherNet, 2009, p. 2). It seems that everyone thinks that the solution to stop bullying is just to educate students about it. I think that it is good that they are trying to educate students about the issue, but we all know that it is going to take more than that to stop bullying. It does not matter how much information you provide the students with about bullying, it still is not going to do anything. People have been trying to stop bullying by just telling students it is not the right thing to do for years and nothing has changed. As you see, they tried this solution in 2007 and now in 2010 the same problem is still going on. You can make students take classes every day about bullying and there will still be children getting bullied. Principals and administrators need to punish students by making use of more suspensions and require mandatory parent meetings for those who are bullying others. If the bullying continues, it must be dealt with strongly. And, both the child and parent should be held responsible for it. Until people start taking actions of this nature, bullying will forever be a problem. I think that the only way to stop bullying is to actually make a law against it. In Massachusetts, they have passed a bill for anti-bullying. I think that is wonderful because now that the law is involved I am sure bullying will be cut down because the kids would not to have to deal with the harsh consequences. â€Å"The bill would prohibit bullying at schools and clamp down on so-called cyber bullying by prohibiting the use of e-mails, text messages, internet postings, and other electronic means to create hostile school environment† (Gay & Lesbians, 2010, p. 16). I also believe that it is good that they are requiring the school principals to report to the bullying to the police, if they believe criminal charges should be pursued. Maybe now if the students know that they could possibly go to jail they would stop bullying. It is great that somebody has passed a bill and takes this issue seriously because this has been going on for too long and it is time for it to stop. Lately there have been many other cities discussing and making laws about bullying. In New Jersey, they have also passed a bill regarding bullying. â€Å"A law against bullying in schools which advocates call the nation’s toughest because it requires schools to develop anti-harassment programs, was approved Monday in New Jersey† (Mulvihill, 2010, pg. ). I believe that this is what the schools need in order for bullying to stop. It may take a while for the schools to get all the policies and programs in the public schools, but it will be worth it. This law will hopefully prevent any more suicides from happening due to children being bullied. Maybe it should have been stricter laws a long time ago and a lot of bullying could have bee n stopped. The state of New Jersey has already passed the law for anti-bullying shows how it will cut down on a lot of bullying. There was already an anti-bullying law in 2002, but it was not required that schools set up anti-bullying programs. The new bill is claimed to be one of the toughest anti-bullying bill that there is. They are going all out the way to make sure students will not get bullied. They are even making teachers get training to help to bulling. Like Julie Bolcer (2010) stated, ‘’ The measure would required training for most public school employees on how to spot bulling and mandate that all districts from ‘school safety teams’ review complaints, reported The Star – Ledger . Superintendent would have to report incidents of buying to the state board of education, which would grade schools and districts on their efforts to combat it ‘’ (pg. 1). I think that it is to stop bulling because this is a very serious problem. But now that there are such harsh laws against it, I know the rates will go down a lot. Bullying is something that has been around for ages, but no one has ever seemed to find the right solution for it. Many say there is not a lot that can be done to stop bulling because it starts off school property; I totally isagree with that. While you have others that think that just by telling students about will stop it, which we all know will not. I feel that the only way bulling will ever stop is if they give harsh punishments and let the bullies know that is not a joke. Even though, there have been many attempts to stop bulling, I think that they have finally found a solution which is by making a tough anti-bulling law. If a child wants to bully someone then they should deal with the law and suffer the consequences.

Business Driven Information Systems

1. Do you agree or disagree with Friedman’s assessment that the world is flat? Be sure to justify your answer. I don’t fully agree with Friedman’s vision of the â€Å"flat World†. Though I think we are more connected and informed than ever. According to me I feel Friedman’s views and research is mostly concerning the developed countries focusing on business and targeting the cooperate world. Today technology, telecommunications has definitely helped people around the world to be closer. But Friedman’s does not mention the less fortunate countries where the globalization is still a far dream. I feel Friedman’s analysis is a good fit for the rich and developed countries, where World has changed to a â€Å"global village†. 2. What are the potential impacts of a flat world for a student performing a job search? Today’s educated youth has all the potential and access to the world of technology at their fingertips. They can easily change to the growing job demands and are ready for job changes as needed. They sure got to have good education as the competition is high in today’s economy. I don’t think it’s difficult to find a job; it depends on how open you are to change in the new world of job opportunities and choose the best option. . What can students do to prepare themselves for competing in a flat world? Students can equip themselves for this competitive World by acquiring good education. Be updated in the field of your study and keep yourself open to news and updates. The coffee shop business is mainly framed in 2 mainstreams. Fi rst group is the major players like Starbucks, Seattle's Best Coffee, Dietrich, Brewsters, New World, Gloria Gears, which can be denoted as Specialty Brands. Second group contains One-Store coffee shops like The Broadway Cafe. These are generally local, small businesses. The coffee shop industry possesses a very high intensified competition on all players: both Specialty Brand and One-Store Coffee Shops. The main reasons that contribute to the high competition are †¢ The number of shops is huge (hoovers. com) †¢ Competitors are selling same or similar products, including specialty coffees as well as high quality foods. †¢ The fixed cost portion constitutes a high portion of the cost structure. Companies should sell more products to cover these costs, which increases competition.

Guinness Corporate Strategy

Income Segmentation although Guinness don't directly segment its product into different segments, Guinness beers are more expensive to the consumer because they target customers may be willing to pay more for what some pensive to be a distinctive taste – a taste which is more expensive to produce. The Japanese distribution system Distribution channels in Japan are very different from other countries; they are as inefficient as they are complex. The system is characterized by multiple layers of wholesalers who have developed close, personal relationships with other wholesalers, manufacturers, importers, and retailers.Moreover, these intimate relationships often serve as an informal barrier to foreign companies wishing to sell directly to end-users or retailers. Many exporters find retailers/end-users unwilling to disrupt their longstanding, personal relationships with Japanese suppliers even when the foreign company can offer a product of superior or equal quality at a cheaper price. Many Japanese retailers/ end-users are unwilling to make the switch to an â€Å"unreliable† foreign supplier. They fear a lack of commitment on the part of the foreign supplier will lead to problems.They also fear breakdowns in communication. This state of affairs has led many companies' new-to-market exporters to complain of the complexity and lack of transparency of the Japanese system. An encouraging sign is the recent trend towards greater efficiency within the Japanese distribution system, resulting in fewer smaller retailers and wholesalers. Faced with deregulation and changing patterns of consumption, many Japanese companies are modifying marketing and sales strategies to take advantage of these developments.Imports are already benefiting from these trends, as seen in increased sales by Japanese department stores and other mass merchandisers and by a variety of new retailing ventures that match changing Japanese lifestyles. There are also indications that some w holesalers are modernizing and consolidating operations, thus reducing more inefficient elements in the system. However, the process is slow. The characteristics of the distribution system are deeply rooted in the cultural history of Japan. How to Set Up Business in Japan/ Laws & Regulations on Setting Up Business in Japan Section 1.Incorporating Your Business . 1 Types of operation in Japan Foreign companies generally establish a business presence in Japan in one of four modes. 1. 1. 1 Representative office Representative offices are established as locations for carrying out preparatory and supplemental tasks aimed at enabling foreign companies to engage in full-scale business operations in Japan. These offices may conduct market surveys, collect information, purchase goods and implement publicity/advertising efforts, but they are not permitted to engage in sales activities.The establishment of representative offices does not require registration. A representative office cannot ord inarily open bank accounts or lease real estate in its own name, so agreements for such purposes must Instead De Selenga Day ten nana Outlet AT ten Torrent company or representative at the representative office in an individual capacity. 1. 1. 2 Branch office Foreign companies wishing to engage in business operations in Japan must establish a branch office or a subsidiary company. The simplest means for a foreign company to establish a base for business operations in Japan is to set up a branch office.The branch office can begin business operations as soon as an office location is secured, he branch office representative determined, and the necessary information registered. A Japanese branch office is a business location that provides services in Japan decided upon by an organization authorized by the foreign company, and ordinarily is not expected to engage in independent decision making. A branch office does not have its own legal corporate status, but instead is deemed to be enco mpassed within the corporate status of the foreign company.In general, therefore, the foreign company is ultimately responsible for all debts and credits generated by the activities of its Japanese branch office. A Japanese branch office, however, may open bank accounts and lease real estate in its own name. 1. 1. 3 Subsidiary company A foreign company establishing a subsidiary company in Japan must choose to establish the subsidiary company as a Joint-stock corporation (Kabuki's-Aisha (K. K. )), limited liability company (Good-Aisha (LLC)), or similar entity stipulated by Japan's Corporate Law.Both unlimited partnerships (Gomes-Aisha) and limited partnerships (Gosh-Aisha) are granted corporate status under the Corporate Law, but they are rarely chosen in practice because equity participants bear unlimited ether than limited liability. All types of subsidiary companies can be established by completing the required procedures stipulated by law and then registering the corporation. A subsidiary is a separate corporation from the foreign company, so the foreign company will bear the liability of an equity participant stipulated by law for all debts and credits generated by the activities of the subsidiary.Other methods by which a foreign company may invest in Japan using a Japanese corporation but without establishing a subsidiary are by establishing a Joint venture with a Japanese enterprise or investment company, and by equity participation in a Japanese enterprise. 1. 1. 4 Limited liability partnership (ALP) It is also possible to do business by using a Huge Seeking Jaggy Kumara. This type of entity, considered the Japanese version of a limited liability partnership (ALP), is not a corporation, but a partnership formed only by the equity participants, who have limited liability.

Industrial Revolutions Essay Example | Topics and Well Written Essays - 500 words

Industrial Revolutions - Essay Example According to Read and LeBlanc, current methods such as intrauterine devices (IUDs) and oral pills have high proven effectiveness (63). These methods reduce the rate of fertility in human beings and limit the likelihood of conception during the period of their consistent use. Some contraceptives such as oral pills, according to Read and LeBlanc can impact the health of newborns, especially if they are used for birth control in extensive period of time (65). Induced abortion limits fertility not by impacting fecundability but by flushing out the pregnancy. The practice has for long been the order of the day in populations and is fairly ordinary in less Christian societies (Read and LeBlanc 68). Read and LeBlanc aver that official statistics of the rates of abortion exceeds 30 percent in countries where the rates are high. Unfortunately, a fairly high number of unrecorded terminations of pregnancies probably take place even in nations reporting very insignificant figures (69). This practice impacts the evolution of populations by denying the unborn an opportunity to live. Complete cutting off of one’s ability to conceive or contribute toward conception impacts evolution of population. The surgical processes of vasectomy and tubal ligation in men and women respectively have become widespread in many countries and cultures. Sterilization denies the affected parties a chance to give birth to children of their own. High death rates among populations, perhaps due to poor health care, and nutrition programs can impact the evolution of population by increasing the likelihood of lower life expectancy. But better health care programs will enhance life expectancy in a particular population. Infant mortality impacts evolution of population by determining the number of infants who will survive to maturity and those who die at birth or immediately after they are born. Improved